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macsquant running buffer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec macsquant running buffer
    Macsquant Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macsquant+running+buffer/MACSQuant+Running+Buffer/pm42508561-81-14-17
    Average 95 stars, based on 89 article reviews
    macsquant running buffer - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: High-throughput screening approach identifies substrate-selective Hsp104 variants that counter amyloid seeding with diminished off-target effects.
    Article Snippet: .. Cells for flow cytometry were fixed in 4% paraformaldehyde (Thermo Fisher) and resuspended in MACSQuant Running Buffer (Miltenyi Biotec) until running on the Miltenyi VYB Flow Cytometer (Miltenyi Biotec). ..

    Article Title: Nanopore Electroporation: A New Delivery Method Within the Field of Epigenetic Editing.
    Article Snippet: The following primer air was used to clone the sgRNA into the MLM3636 vector Plasmid #43860, Addgene, Watertown, MA, USA) as previously escribed (Primer sense (Sequence 5′-3′): ACACCGCTTTGCGTTTGGCCCATTAG and Primer antisense (Sequence 5′-3′): AAACTAATGGGCCAAACGGCAAAGCG) [52]. of 11 .. After resuspending INS1 β-cells according to the passaging protocol, the cell suspension was diluted 10 times in DPBS containing DAPI (0.01 μg/ml final concentration) before determination of cell concentration and viability using flow cytometry on a MACSQuant Analyzer 16 Flow cytometer with MACSQuant running buffer, storage solution, and washing solution (Miltenyi Biotech, Bergisch Gladbach, Germany). .. For the optimization of nanopore electroporation, the dCas9KRAB plasmid (0.2 μg/μl in MilliQ water) was stained with the intercalating dye YOYO-1 iodide (Invitrogen, ThermoFisher).

    CMC:

    Article Title: Dual pH-Responsive Calcium Phosphate Nanoparticles Conjugated with Folate by CuAAC Click Chemistry for Targeted Gemcitabine Delivery to Cancer Cells
    Article Snippet: .. The following reagents were used: carboxymethylcellulose sodium salt (CMC; DS 0.7, M w ∼ 90 kDa, Sigma-Aldrich), 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC; ≥99%, Carl Roth), N -hydroxysuccinimide (NHS; 98%, Sigma-Aldrich), gemcitabine hydrochloride (GEM; >98%, TCI), calcium lactate pentahydrate (USP Reference Standard, Sigma-Aldrich), ammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ; ≥98%, VWR Life Science), tetraethyl orthosilicate (TEOS; 98%, Merck), CMC-BR (label degree 1:66, M w ∼ 200 kDa, Surflay Nanotec), (3-azidopropyl) triethoxysilane (97%, SelectLab Chemicals), ( S )-17-(4-(((2-amino-4-oxo-3,4-dihydropteridin-6-yl) methyl) amino) benzamido)-14-oxo-4,7,10-trioxa-13-azaoctadec-1-yn-18-oic acid (Folate-PEG3-propargyl; 97%, AmBeed), copper sulfate pentahydrate (CuSO 4 ·5H 2 O; 99%, AppliChem), tris (3-hydroxypropyl-triazolylmethyl) amine (THPTA; 95%, Sigma-Aldrich), aminoguanidine hydrogen carbonate (98+%, Alfa Aesar), sodium ascorbate (≥99%, Sigma-Aldrich), potassium bromide (KBr; Sigma-Aldrich), dimethylformamide (DMF; Fisher Scientific), dimethyl sulfoxide (DMSO; Fisher Scientific), absolute ethanol (Fisher Scientific), ammonia solution (30%, Carl Roth), sodium hydroxide (NaOH; 0.1 M, Bernd Kraft), glacial acetic acid (Fisher Scientific), deuterium oxide (D 2 O; 99.9%, Deutero), chloride acid solution (HCl; 1 M, Bernd Kraft), Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific), fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific), RPMI 1640 (Gibco, Thermo Fisher Scientific), penicillin (Gibco, Thermo Fisher Scientific), streptomycin (Gibco, Thermo Fisher Scientific), GlutaMAX (Gibco, Thermo Fisher Scientific), Dulbecco’s phosphate-buffered saline (DPBS; Gibco, Thermo Fisher Scientific), TrypLE Express Enzyme (Gibco, Thermo Fisher Scientific), hexamethyldisilazane (HMDS; Sigma-Aldrich), MACSQuant running buffer (Miltenyi Biotec), paraformaldehyde (PFA; p.a., Merck), AF488 phalloidin conjugate (AAT Bioquest, Biomol), Hoechst 33342 (Life Technologies), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, Thermo Fisher Scientific). ..

    Modification:

    Article Title: Dual pH-Responsive Calcium Phosphate Nanoparticles Conjugated with Folate by CuAAC Click Chemistry for Targeted Gemcitabine Delivery to Cancer Cells
    Article Snippet: .. The following reagents were used: carboxymethylcellulose sodium salt (CMC; DS 0.7, M w ∼ 90 kDa, Sigma-Aldrich), 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC; ≥99%, Carl Roth), N -hydroxysuccinimide (NHS; 98%, Sigma-Aldrich), gemcitabine hydrochloride (GEM; >98%, TCI), calcium lactate pentahydrate (USP Reference Standard, Sigma-Aldrich), ammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ; ≥98%, VWR Life Science), tetraethyl orthosilicate (TEOS; 98%, Merck), CMC-BR (label degree 1:66, M w ∼ 200 kDa, Surflay Nanotec), (3-azidopropyl) triethoxysilane (97%, SelectLab Chemicals), ( S )-17-(4-(((2-amino-4-oxo-3,4-dihydropteridin-6-yl) methyl) amino) benzamido)-14-oxo-4,7,10-trioxa-13-azaoctadec-1-yn-18-oic acid (Folate-PEG3-propargyl; 97%, AmBeed), copper sulfate pentahydrate (CuSO 4 ·5H 2 O; 99%, AppliChem), tris (3-hydroxypropyl-triazolylmethyl) amine (THPTA; 95%, Sigma-Aldrich), aminoguanidine hydrogen carbonate (98+%, Alfa Aesar), sodium ascorbate (≥99%, Sigma-Aldrich), potassium bromide (KBr; Sigma-Aldrich), dimethylformamide (DMF; Fisher Scientific), dimethyl sulfoxide (DMSO; Fisher Scientific), absolute ethanol (Fisher Scientific), ammonia solution (30%, Carl Roth), sodium hydroxide (NaOH; 0.1 M, Bernd Kraft), glacial acetic acid (Fisher Scientific), deuterium oxide (D 2 O; 99.9%, Deutero), chloride acid solution (HCl; 1 M, Bernd Kraft), Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific), fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific), RPMI 1640 (Gibco, Thermo Fisher Scientific), penicillin (Gibco, Thermo Fisher Scientific), streptomycin (Gibco, Thermo Fisher Scientific), GlutaMAX (Gibco, Thermo Fisher Scientific), Dulbecco’s phosphate-buffered saline (DPBS; Gibco, Thermo Fisher Scientific), TrypLE Express Enzyme (Gibco, Thermo Fisher Scientific), hexamethyldisilazane (HMDS; Sigma-Aldrich), MACSQuant running buffer (Miltenyi Biotec), paraformaldehyde (PFA; p.a., Merck), AF488 phalloidin conjugate (AAT Bioquest, Biomol), Hoechst 33342 (Life Technologies), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, Thermo Fisher Scientific). ..

    Saline:

    Article Title: Dual pH-Responsive Calcium Phosphate Nanoparticles Conjugated with Folate by CuAAC Click Chemistry for Targeted Gemcitabine Delivery to Cancer Cells
    Article Snippet: .. The following reagents were used: carboxymethylcellulose sodium salt (CMC; DS 0.7, M w ∼ 90 kDa, Sigma-Aldrich), 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC; ≥99%, Carl Roth), N -hydroxysuccinimide (NHS; 98%, Sigma-Aldrich), gemcitabine hydrochloride (GEM; >98%, TCI), calcium lactate pentahydrate (USP Reference Standard, Sigma-Aldrich), ammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ; ≥98%, VWR Life Science), tetraethyl orthosilicate (TEOS; 98%, Merck), CMC-BR (label degree 1:66, M w ∼ 200 kDa, Surflay Nanotec), (3-azidopropyl) triethoxysilane (97%, SelectLab Chemicals), ( S )-17-(4-(((2-amino-4-oxo-3,4-dihydropteridin-6-yl) methyl) amino) benzamido)-14-oxo-4,7,10-trioxa-13-azaoctadec-1-yn-18-oic acid (Folate-PEG3-propargyl; 97%, AmBeed), copper sulfate pentahydrate (CuSO 4 ·5H 2 O; 99%, AppliChem), tris (3-hydroxypropyl-triazolylmethyl) amine (THPTA; 95%, Sigma-Aldrich), aminoguanidine hydrogen carbonate (98+%, Alfa Aesar), sodium ascorbate (≥99%, Sigma-Aldrich), potassium bromide (KBr; Sigma-Aldrich), dimethylformamide (DMF; Fisher Scientific), dimethyl sulfoxide (DMSO; Fisher Scientific), absolute ethanol (Fisher Scientific), ammonia solution (30%, Carl Roth), sodium hydroxide (NaOH; 0.1 M, Bernd Kraft), glacial acetic acid (Fisher Scientific), deuterium oxide (D 2 O; 99.9%, Deutero), chloride acid solution (HCl; 1 M, Bernd Kraft), Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific), fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific), RPMI 1640 (Gibco, Thermo Fisher Scientific), penicillin (Gibco, Thermo Fisher Scientific), streptomycin (Gibco, Thermo Fisher Scientific), GlutaMAX (Gibco, Thermo Fisher Scientific), Dulbecco’s phosphate-buffered saline (DPBS; Gibco, Thermo Fisher Scientific), TrypLE Express Enzyme (Gibco, Thermo Fisher Scientific), hexamethyldisilazane (HMDS; Sigma-Aldrich), MACSQuant running buffer (Miltenyi Biotec), paraformaldehyde (PFA; p.a., Merck), AF488 phalloidin conjugate (AAT Bioquest, Biomol), Hoechst 33342 (Life Technologies), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, Thermo Fisher Scientific). ..

    Article Title: A Replication-Competent Flavivirus Genome with a Stable GFP Insertion at the NS1-NS2A Junction.
    Article Snippet: .. Cells were detached using trypsin-EDTA, washed with phosphate-buffered saline (PBS), resuspended in MACSQuant Running Buffer (Miltenyi Biotec Cat. 130-092-747, Bergisch Gladbach, Germany) and passed through a 70 μm strainer. .. Samples were analyzed on a MACS Quant Analyzer 10 flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: A Replication-Competent Flavivirus Genome with a Stable GFP Insertion at the NS1-NS2A Junction
    Article Snippet: .. Cells were detached using trypsin-EDTA, washed with phosphate-buffered saline (PBS), resuspended in MACSQuant Running Buffer (Miltenyi Biotec Cat. 130-092-747, Bergisch Gladbach, Germany) and passed through a 70 μm strainer. .. Samples were analyzed on a MACS Quant Analyzer 10 flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany).

    MTT Assay:

    Article Title: Dual pH-Responsive Calcium Phosphate Nanoparticles Conjugated with Folate by CuAAC Click Chemistry for Targeted Gemcitabine Delivery to Cancer Cells
    Article Snippet: .. The following reagents were used: carboxymethylcellulose sodium salt (CMC; DS 0.7, M w ∼ 90 kDa, Sigma-Aldrich), 1-ethyl-3-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC; ≥99%, Carl Roth), N -hydroxysuccinimide (NHS; 98%, Sigma-Aldrich), gemcitabine hydrochloride (GEM; >98%, TCI), calcium lactate pentahydrate (USP Reference Standard, Sigma-Aldrich), ammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ; ≥98%, VWR Life Science), tetraethyl orthosilicate (TEOS; 98%, Merck), CMC-BR (label degree 1:66, M w ∼ 200 kDa, Surflay Nanotec), (3-azidopropyl) triethoxysilane (97%, SelectLab Chemicals), ( S )-17-(4-(((2-amino-4-oxo-3,4-dihydropteridin-6-yl) methyl) amino) benzamido)-14-oxo-4,7,10-trioxa-13-azaoctadec-1-yn-18-oic acid (Folate-PEG3-propargyl; 97%, AmBeed), copper sulfate pentahydrate (CuSO 4 ·5H 2 O; 99%, AppliChem), tris (3-hydroxypropyl-triazolylmethyl) amine (THPTA; 95%, Sigma-Aldrich), aminoguanidine hydrogen carbonate (98+%, Alfa Aesar), sodium ascorbate (≥99%, Sigma-Aldrich), potassium bromide (KBr; Sigma-Aldrich), dimethylformamide (DMF; Fisher Scientific), dimethyl sulfoxide (DMSO; Fisher Scientific), absolute ethanol (Fisher Scientific), ammonia solution (30%, Carl Roth), sodium hydroxide (NaOH; 0.1 M, Bernd Kraft), glacial acetic acid (Fisher Scientific), deuterium oxide (D 2 O; 99.9%, Deutero), chloride acid solution (HCl; 1 M, Bernd Kraft), Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific), fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific), RPMI 1640 (Gibco, Thermo Fisher Scientific), penicillin (Gibco, Thermo Fisher Scientific), streptomycin (Gibco, Thermo Fisher Scientific), GlutaMAX (Gibco, Thermo Fisher Scientific), Dulbecco’s phosphate-buffered saline (DPBS; Gibco, Thermo Fisher Scientific), TrypLE Express Enzyme (Gibco, Thermo Fisher Scientific), hexamethyldisilazane (HMDS; Sigma-Aldrich), MACSQuant running buffer (Miltenyi Biotec), paraformaldehyde (PFA; p.a., Merck), AF488 phalloidin conjugate (AAT Bioquest, Biomol), Hoechst 33342 (Life Technologies), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, Thermo Fisher Scientific). ..

    Passaging:

    Article Title: Nanopore Electroporation: A New Delivery Method Within the Field of Epigenetic Editing.
    Article Snippet: The following primer air was used to clone the sgRNA into the MLM3636 vector Plasmid #43860, Addgene, Watertown, MA, USA) as previously escribed (Primer sense (Sequence 5′-3′): ACACCGCTTTGCGTTTGGCCCATTAG and Primer antisense (Sequence 5′-3′): AAACTAATGGGCCAAACGGCAAAGCG) [52]. of 11 .. After resuspending INS1 β-cells according to the passaging protocol, the cell suspension was diluted 10 times in DPBS containing DAPI (0.01 μg/ml final concentration) before determination of cell concentration and viability using flow cytometry on a MACSQuant Analyzer 16 Flow cytometer with MACSQuant running buffer, storage solution, and washing solution (Miltenyi Biotech, Bergisch Gladbach, Germany). .. For the optimization of nanopore electroporation, the dCas9KRAB plasmid (0.2 μg/μl in MilliQ water) was stained with the intercalating dye YOYO-1 iodide (Invitrogen, ThermoFisher).

    Suspension:

    Article Title: Nanopore Electroporation: A New Delivery Method Within the Field of Epigenetic Editing.
    Article Snippet: The following primer air was used to clone the sgRNA into the MLM3636 vector Plasmid #43860, Addgene, Watertown, MA, USA) as previously escribed (Primer sense (Sequence 5′-3′): ACACCGCTTTGCGTTTGGCCCATTAG and Primer antisense (Sequence 5′-3′): AAACTAATGGGCCAAACGGCAAAGCG) [52]. of 11 .. After resuspending INS1 β-cells according to the passaging protocol, the cell suspension was diluted 10 times in DPBS containing DAPI (0.01 μg/ml final concentration) before determination of cell concentration and viability using flow cytometry on a MACSQuant Analyzer 16 Flow cytometer with MACSQuant running buffer, storage solution, and washing solution (Miltenyi Biotech, Bergisch Gladbach, Germany). .. For the optimization of nanopore electroporation, the dCas9KRAB plasmid (0.2 μg/μl in MilliQ water) was stained with the intercalating dye YOYO-1 iodide (Invitrogen, ThermoFisher).

    Concentration Assay:

    Article Title: Nanopore Electroporation: A New Delivery Method Within the Field of Epigenetic Editing.
    Article Snippet: The following primer air was used to clone the sgRNA into the MLM3636 vector Plasmid #43860, Addgene, Watertown, MA, USA) as previously escribed (Primer sense (Sequence 5′-3′): ACACCGCTTTGCGTTTGGCCCATTAG and Primer antisense (Sequence 5′-3′): AAACTAATGGGCCAAACGGCAAAGCG) [52]. of 11 .. After resuspending INS1 β-cells according to the passaging protocol, the cell suspension was diluted 10 times in DPBS containing DAPI (0.01 μg/ml final concentration) before determination of cell concentration and viability using flow cytometry on a MACSQuant Analyzer 16 Flow cytometer with MACSQuant running buffer, storage solution, and washing solution (Miltenyi Biotech, Bergisch Gladbach, Germany). .. For the optimization of nanopore electroporation, the dCas9KRAB plasmid (0.2 μg/μl in MilliQ water) was stained with the intercalating dye YOYO-1 iodide (Invitrogen, ThermoFisher).



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    Miltenyi Biotec macsquant running buffer for cytometry
    Production and titration of LV using replicon cell pools (RCPs) or conventional three-plasmid transfection of HEK293FT cells. ( a ) LV/CAR-GFP production in 10 cm dishes. Supernatant was harvested at 48 h post-transfection, replaced with fresh medium, and collected again at 96 h. Bar graph shows functional titers (TU/mL) from three independent experiments (mean ± SD). ( b ) Representative images of HEK293FT cells transduced with LV/CAR-GFP during titration experiment. Cells in the first well of a 6-well plate (1:10 dilution of culture supernatant) were photographed 48 h post-transduction. Left: phase contrast; right: GFP fluorescence. Magnification: 10× objective; scale bar: 100 µm. ( c ) Schematic workflow of large-scale LV/CAR production in 5-layer stacks using RCP cells, or conventional HEK293FT cells. ( d ) Flow <t>cytometry</t> histograms for titration of LV/CAR vector. HEK293FT cells were transduced to express CAR and stained with PE-conjugated anti-CAR antibodies. Numbers indicate the percentage of CAR-positive cells.
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    Image Search Results


    Production and titration of LV using replicon cell pools (RCPs) or conventional three-plasmid transfection of HEK293FT cells. ( a ) LV/CAR-GFP production in 10 cm dishes. Supernatant was harvested at 48 h post-transfection, replaced with fresh medium, and collected again at 96 h. Bar graph shows functional titers (TU/mL) from three independent experiments (mean ± SD). ( b ) Representative images of HEK293FT cells transduced with LV/CAR-GFP during titration experiment. Cells in the first well of a 6-well plate (1:10 dilution of culture supernatant) were photographed 48 h post-transduction. Left: phase contrast; right: GFP fluorescence. Magnification: 10× objective; scale bar: 100 µm. ( c ) Schematic workflow of large-scale LV/CAR production in 5-layer stacks using RCP cells, or conventional HEK293FT cells. ( d ) Flow cytometry histograms for titration of LV/CAR vector. HEK293FT cells were transduced to express CAR and stained with PE-conjugated anti-CAR antibodies. Numbers indicate the percentage of CAR-positive cells.

    Journal: Biology

    Article Title: Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production

    doi: 10.3390/biology15110848

    Figure Lengend Snippet: Production and titration of LV using replicon cell pools (RCPs) or conventional three-plasmid transfection of HEK293FT cells. ( a ) LV/CAR-GFP production in 10 cm dishes. Supernatant was harvested at 48 h post-transfection, replaced with fresh medium, and collected again at 96 h. Bar graph shows functional titers (TU/mL) from three independent experiments (mean ± SD). ( b ) Representative images of HEK293FT cells transduced with LV/CAR-GFP during titration experiment. Cells in the first well of a 6-well plate (1:10 dilution of culture supernatant) were photographed 48 h post-transduction. Left: phase contrast; right: GFP fluorescence. Magnification: 10× objective; scale bar: 100 µm. ( c ) Schematic workflow of large-scale LV/CAR production in 5-layer stacks using RCP cells, or conventional HEK293FT cells. ( d ) Flow cytometry histograms for titration of LV/CAR vector. HEK293FT cells were transduced to express CAR and stained with PE-conjugated anti-CAR antibodies. Numbers indicate the percentage of CAR-positive cells.

    Article Snippet: Cells were detached with trypsin-EDTA, washed with PBS, and resuspended in 1 mL of MACSQuant Running Buffer for cytometry (RB, Miltenyi Biotec Cat. 130-092-747).

    Techniques: Titration, Plasmid Preparation, Transfection, Functional Assay, Transduction, Fluorescence, Flow Cytometry, Staining

    Characterization of a CAR-T cell product generated using LV/CAR vector produced from RCP cells. ( a ) Representative flow cytometry plots showing the gating strategy used for counting CAR + cells (debris exclusion, CD45 + leukocytes, viable cells, CD3 + T cells, and CAR + subset within CD3 + cells). ( b ) Summary of cell product composition after T-cell selection and transduction, including cell subsets, markers, event counts, and relative frequencies.

    Journal: Biology

    Article Title: Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production

    doi: 10.3390/biology15110848

    Figure Lengend Snippet: Characterization of a CAR-T cell product generated using LV/CAR vector produced from RCP cells. ( a ) Representative flow cytometry plots showing the gating strategy used for counting CAR + cells (debris exclusion, CD45 + leukocytes, viable cells, CD3 + T cells, and CAR + subset within CD3 + cells). ( b ) Summary of cell product composition after T-cell selection and transduction, including cell subsets, markers, event counts, and relative frequencies.

    Article Snippet: Cells were detached with trypsin-EDTA, washed with PBS, and resuspended in 1 mL of MACSQuant Running Buffer for cytometry (RB, Miltenyi Biotec Cat. 130-092-747).

    Techniques: Generated, Plasmid Preparation, Produced, Flow Cytometry, Selection, Transduction